Toluidine blue stain
Toluidine blue stain in a vasculitic peripheral neuropathy.
Toluidine blue is a basic thiazine metachromatic dye with high affinity for acidic tissue components.[1]
It stains nucleic acids blue and polysaccharides purple and also increases the sharpness of histology slide images. It is especially useful today for staining chromosomes in plant or animal tissues, as a replacement for Aceto-orcein stain.
Results
The results depend on the studied organs:[2]
- Mastocytes in purple
- Cartilage in purple
- Mucins in purple/red
- Nuclei in blue
It is used in forensic examination,[3] renal pathology[4] and neuropathology.
See also
References
- ↑ Sridharan, G; Shankar, AA (2012). "Toluidine blue: A review of its chemistry and clinical utility". J Oral Maxillofac Pathol. 16: 251–5. doi:10.4103/0973-029X.99081. PMC 3424943
. PMID 22923899. - ↑ http://www.histalim.com/accueil/activities/our-services/histology/toluidine-blue/
- ↑ Olshaker, Jackson and Smock (2001). Forensic Emergency Medicine. Philadelphia: Lippincott, Williams and Williams. pp. 94–97. ISBN 0781731445.
- ↑ Nicholas, SB.; Basgen, JM.; Sinha, S. (2011). "Using stereologic techniques for podocyte counting in the mouse: shifting the paradigm.". Am J Nephrol. 33 Suppl 1: 1–7. doi:10.1159/000327564. PMC 3121548. PMID 21659728.
External links
- Sridharan, G; Shankar, AA (2012). "Toluidine blue: A review of its chemistry and clinical utility". J Oral Maxillofac Pathol. 16: 251–5. doi:10.4103/0973-029X.99081. PMC 3424943. PMID 22923899.
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