Pierce Protein Assay
Pierce Protein Assay is a method of quantification of protein. It provides quick estimation of protein amount in sample.[1]
Protocol
The assay is separated into three main parts:
- Prepare the Diluted Albumin (BSA) Standards.
- Prepare the bicinchoninic acid (BCA) working reagent (WR).
- Quantification of proteins (depending on selected procedure either test-tube procedure or microplate procedure)
Advantages of Pierce Method
- Able to detect as low as 25 ug/ml and up to 2000 ug/ml proteins in 65 ul sample in standard protocol.
- A preferred methods for samples containing detergents or other reducing agents.
- Fast detection in comparison to the BCA or Coomassie (Bradford) Assays.
- A better approach compared to Coomassie (Bradford) Assay due to low protein-to-protein variability.
- Have stable end point.
Disadvantages of Pierce Method
- Protein-to-protein have greater variability than the BCA Assay.
Analytical reagents and tests | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Metals | |||||||||||
| Sugars, fats, and proteins |
| ||||||||||
| Alcohols |
| ||||||||||
| Drugs |
| ||||||||||
| other |
| ||||||||||
.svg.png){kind=small}
References
- ↑ "Protein Assay Handbook" (PDF). Life technologies. Retrieved 9 April 2015.
This article is issued from Wikipedia - version of the 4/30/2016. The text is available under the Creative Commons Attribution/Share Alike but additional terms may apply for the media files.