Grocott's methenamine silver stain

A liver biopsy stained using the grocott's methenamine silver stain demonstrating histoplasma (black round balls) in a granuloma.

In pathology, the Grocott-Gomori's (or Gömöri) methenamine silver stain, abbreviated GMS, is a popular staining method in histology.

It is used widely as a screen for fungal organisms. Particularly useful in staining carbohydrates.

It can be used to identify the yeast-like fungus Pneumocystis jiroveci^[1] which causes a form of pneumonia called Pneumocystis Pneumonia (PCP) or Pneumocystosis.

The cell walls of these organisms are outlined by the brown to black stain.

References

↑ Nassar A, Zapata M, Little JV, Siddiqui MT (November 2006). "Utility of reflex gomori methenamine silver staining for Pneumocystis jirovecii on bronchoalveolar lavage cytologic specimens: A review". Diagn. Cytopathol. 34 (11): 719–23. doi:10.1002/dc.20540. PMID 17041954.

Microbial and histological stains

Iron/hemosiderin	Prussian blue

Lipids	Sudan stain Sudan II Sudan III Sudan IV Oil Red O Sudan Black B

Carbohydrates	Periodic acid–Schiff stain

Amyloid	Congo red

Bacteria	Gram staining Methyl violet/Gentian violet Safranin Ziehl–Neelsen stain/acid-fast Carbol fuchsin/Fuchsine Methylene blue Auramine-rhodamine stain Auramine O Rhodamine B

Connective tissue	trichrome stain: Masson's trichrome stain/Lillie's trichrome Light Green SF yellowish Biebrich scarlet Phosphomolybdic acid Fast Green FCF Van Gieson's stain

Other	H&E stain Haematoxylin Eosin Y Silver stain Grocott's methenamine silver stain Warthin–Starry stain Methyl blue Wright's stain Giemsa stain Gömöri trichrome stain Neutral red Janus Green B Alcian blue stain Movat's stain

Tissue stainability	Acidophilic Basophilic Chromophobic

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