Crossover junction endodeoxyribonuclease
| Crossover junction endodeoxyribonuclease | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC number | 3.1.22.4 | ||||||||
| CAS number | 99676-43-4 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| |||||||||
Crossover junction endodeoxyribonuclease (EC 3.1.22.4, Hje endonuclease, Holliday junction endonuclease CCE1, Holliday junction resolvase, Holliday junction-cleaving endonuclease, Holliday junction-resolving endoribonuclease, RusA Holliday junction resolvase, RusA endonuclease, RuvC endonuclease, SpCCe1 Holliday junction resolvase, crossover junction endoribonuclease, cruciform-cutting endonuclease, endo X3, endonuclease RuvC, endonuclease VII, endonuclease X3, resolving enzyme CCE1) is an enzyme.[1][2][3][4][5] This enzyme catalyses the following chemical reaction
- Endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction)
The enzyme from Saccharomyces cerevisiae is not active on DNA molecules that do not contain Holliday junctions.
References
- ↑ Symington, L.S.; Kolodner, R. (1985). "Partial purification of an enzyme from Saccharomyces cerevisiae that cleaves Holliday junctions". Proc. Natl. Acad. Sci. USA. 82: 7247–7251. doi:10.1073/pnas.82.21.7247. PMID 3903750.
- ↑ Shida, T.; Iwasaki, H.; Saito, A.; Kyogoku, Y.; Shinagawa, H. (1996). "Analysis of substrate specificity of the RuvC holliday junction resolvase with synthetic Holliday junctions". J. Biol. Chem. 271: 26105–26109. doi:10.1074/jbc.271.42.26105. PMID 8824253.
- ↑ Shah, R.; Cosstick, R.; West, S.C. (1997). "The RuvC protein dimer resolves Holliday junctions by a dual incision mechanism that involves base-specific contacts". EMBO J. 16: 1464–1472. doi:10.1093/emboj/16.6.1464. PMID 9135161.
- ↑ Fogg, J.M.; Schofield, M.J.; White, M.F.; Lilley, D.M. (1999). "Sequence and functional-group specificity for cleavage of DNA junctions by RuvC of Escherichia coli". Biochemistry. 38: 11349–11358. doi:10.1021/bi990926n. PMID 10471285.
- ↑ Middleton, C.L.; Parker, J.L.; Richard, D.J.; White, M.F.; Bond, C.S. (2003). "Crystallization and preliminary X-ray diffraction studies of Hje, a Holliday junction resolving enzyme from Sulfolobus solfataricus". Acta Crystallogr. D. 59: 171–173. doi:10.1107/s0907444902019546. PMID 12499561.
External links
- Crossover junction endodeoxyribonuclease at the US National Library of Medicine Medical Subject Headings (MeSH)
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