Chloroquine

Chloroquine

Clinical data
Pronunciation	/ˈklɔːrəkwɪn/
Trade names	Aralen
AHFS/Drugs.com	Monograph
License data	US FDA: Chloroquine
ATC code	P01BA01 (WHO)
Legal status
Legal status	US: ℞-only
Pharmacokinetic data
Metabolism	Liver
Biological half-life	1–2 months
Identifiers
IUPAC name (RS)-N'-(7-chloroquinolin-4-yl)-N,N-diethyl-pentane-1,4-diamine
CAS Number	54-05-7 Y
PubChem (CID)	2719
IUPHAR/BPS	5535
DrugBank	DB00608 Y
ChemSpider	2618 Y
UNII	886U3H6UFF Y
KEGG	D02366 Y
ChEBI	CHEBI:3638 Y
ChEMBL	CHEMBL76 Y
NIAID ChemDB	000733
ECHA InfoCard	100.000.175
Chemical and physical data
Formula	C18H26ClN3
Molar mass	319.872 g/mol
3D model (Jmol)	Interactive image
SMILES Clc1cc2nccc(c2cc1)NC(C)CCCN(CC)CC
InChI InChI=1S/C18H26ClN3/c1-4-22(5-2)12-6-7-14(3)21-17-10-11-20-18-13-15(19)8-9-16(17)18/h8-11,13-14H,4-7,12H2,1-3H3,(H,20,21) Y Key:WHTVZRBIWZFKQO-UHFFFAOYSA-N Y
(verify)

Chloroquine is a medication used to prevent and to treat malaria in areas where malaria is known to be sensitive to its effects.^[1] Certain types of malaria, resistant strains, and complicated cases typically require different or additional medication. It is also occasionally used for amebiasis that is occurring outside of the intestines, rheumatoid arthritis, and lupus erythematosus. It is taken by mouth.^[1]

Common side effects include muscle problems, loss of appetite, diarrhea, and skin rash. Serious side effects include problems with vision, muscle damage, seizures, and low blood cell levels. It appears to be safe for use during pregnancy but has not been well studied in this group of people. Chloroquine is a member of the drug class 4-aminoquinoline. It works against the asexual form of malaria inside the red blood cell.^[1]

Chloroquine was discovered in 1934 by Hans Andersag.^[2][3] It is on the World Health Organization's List of Essential Medicines, the most important medications needed in a basic health system.^[4] It is available as a generic medication.^[1] The wholesale cost in the developing world is about 0.04 USD.^[5] In the United States it costs about 5.30 USD per dose.^[1]

Medical uses

Resochin tablet package

Malaria prevention

Chloroquine has long been used in the treatment or prevention of malaria from Plasmodium vivax, P. ovale, and P. malariae. After the malaria parasite Plasmodium falciparum started to develop widespread resistance to it.^[6][7]

Chloroquine has been extensively used in mass drug administrations, which may have contributed to the emergence and spread of resistance. It is recommended to check if chloroquine is still effective in the region prior to using it.^[8] In areas where resistance is present, other antimalarials, such as mefloquine or atovaquone, may be used instead. The Centers for Disease Control and Prevention recommend against treatment of malaria with chloroquine alone due to more effective combinations.^[9]

Amebiasis

In treatment of amoebic liver abscess, chloroquine may be used instead of or in addition to other medications in the event of failure of improvement with metronidazole or another nitroimidazole within 5 days or intolerance to metronidazole or a nitroimidazole.^[10]

Rheumatic disease

As it mildly suppresses the immune system, it is used in some autoimmune disorders, such as rheumatoid arthritis and lupus erythematosus.^[1]

Adverse effects

Side effects include neuromuscular, hearing, gastrointestinal, brain, skin, eye, cardiovascular (rare), and blood reactions.^[11]

• Seizures^[11]
• Hear reactions: deafness or tinnitus.^[11]
• Gastrointestinal reactions: nausea, vomiting, diarrhea, abdominal cramps, and anorexia.^[11]
• Brain reactions: mild and transient headache.^[11]
• Skin reactions: itchiness, skin color changes, hair loss, and skin rashes.^[11]
  • Chloroquine-induced itching is very common among black Africans (70%), but much less common in other races. It increases with age, and is so severe as to stop compliance with drug therapy. It is increased during malaria fever; its severity is correlated to the malaria parasite load in blood. Some evidence indicates it has a genetic basis and is related to chloroquine action with opiate receptors centrally or peripherally.^[12]
• Unpleasant metallic taste
  • This could be avoided by ‘taste-masked and controlled release’ formulations such as multiple emulsions.^[13]
• Eye reactions: chloroquine retinopathy
  • May be irreversible.^[11] This occurs with long-term use over many years or with high doses. Patients on long-term chloroquine therapy should be screened at baseline and then annually after five years of use.^[14] The daily safe maximum doses for eye toxicity can be computed from one's height and weight using this calculator.^[15] Patients should be screened for vision changes such as blurring of vision, difficulty focusing, or seeing half an object.^[11]
• Heart reactions: rare hypotension and electrocardiographic changes^[11][16]
  • This manifests itself as either conduction disturbances (bundle-branch block, atrioventricular block) or cardiomyopathy – often with hypertrophy, restrictive physiology, and congestive heart failure. The changes may be irreversible. Only two cases have been reported requiring heart transplantation, suggesting this particular risk is very low. Electron microscopy of cardiac biopsies show pathognomonic cytoplasmic inclusion bodies.
• Blood reaction: rare pancytopenia, aplastic anemia, reversible agranulocytosis, low blood platlets, neutropenia.^[17]

Pregnancy

Chloroquine has not been shown to have any harmful effects on the fetus when used for malarial prophylaxis.^[18] Small amounts of chloroquine are excreted in the breast milk of lactating women. However, because this drug can be safely prescribed to infants, the effects are not harmful. Studies with mice show that radioactively tagged chloroquine passed through the placenta rapidly and accumulated in the fetal eyes which remained present five months after the drug was cleared from the rest of the body.^[17][19] It is still advised to prevent women who are pregnant or planning on getting pregnant from traveling to malaria-risk regions.^[18]

Elderly

There is not enough evidence to determine whether chloroquine is safe to be given to people aged 65 and older. However, the drug is cleared by the kidneys and toxicity should be monitored carefully in people with poor kidney functions.^[17]

Drug interactions

• Antacids- may reduce absorption of chloroquine; take 4 hours apart
• Kaolin- may reduce absorption of chloroquine; take 4 hours apart
• Cimetidine- may inhibit metabolism of chloroquine; increasing levels of chloroquine in the body
• Ampicillin- levels may be reduced by chloroquine; take 2 hours apart
• Cyclosporine- levels may be increased by chloroquine
• Mefloquine- may increase risk of convulsions^[17]

Overdose

Chloroquine is very dangerous in overdose. It is rapidly absorbed from the gut. In 1961, published studies showed three children who took overdoses died within 2.5 hours of taking the drug. While the amount of the overdose was not cited, the therapeutic index for chloroquine is known to be small.^[20] Symptoms of overdose include headache, drowsiness, visual disturbances, nausea and vomiting, cardiovascular collapse, seizures, and sudden respiratory and cardiac arrest.^[17]

A metabolite of chloroquine – hydroxycloroquine – has a long half-life (32–56 days) in blood and a large volume of distribution (580–815 L/kg).^[21] The therapeutic, toxic and lethal ranges are usually considered to be 0.03 to 15 mg/l, 3.0 to 26 mg/l and 20 to 104 mg/l, respectively. However, nontoxic cases have been reported in the range 0.3 to 39 mg/l, suggesting individual tolerance to this agent may be more variable than previously recognised.^[21]

Resistance in malaria

Since the first documentation of P. falciparum chloroquine resistance in the 1950s, resistant strains have appeared throughout East and West Africa, Southeast Asia, and South America. The effectiveness of chloroquine against P. falciparum has declined as resistant strains of the parasite evolved. They effectively neutralize the drug via a mechanism that drains chloroquine away from the digestive vacuole. Chloroquine-resistant cells efflux chloroquine at 40 times the rate of chloroquine-sensitive cells; the related mutations trace back to transmembrane proteins of the digestive vacuole, including sets of critical mutations in the P. falciparum chloroquine resistance transporter (PfCRT) gene. The mutated protein, but not the wild-type transporter, transports chloroquine when expressed in Xenopus oocytes and is thought to mediate chloroquine leak from its site of action in the digestive vacuole.^[22] Resistant parasites also frequently have mutated products of the ABC transporter P. falciparum multidrug resistance (PfMDR1) gene, although these mutations are thought to be of secondary importance compared to Pfcrt. Verapamil, a Ca²⁺ channel blocker, has been found to restore both the chloroquine concentration ability and sensitivity to this drug. Recently, an altered chloroquine-transporter protein CG2 of the parasite has been related to chloroquine resistance, but other mechanisms of resistance also appear to be involved.^[23] Research on the mechanism of chloroquine and how the parasite has acquired chloroquine resistance is still ongoing, as other mechanisms of resistance are likely.

Other agents which have been shown to reverse chloroquine resistance in malaria are chlorpheniramine, gefitinib, imatinib, tariquidar and zosuquidar.^[24]

Pharmacology

• Absorption: Rapid and almost completely
• Distribution: Widely distributed into body tissues
• Protein binding: 55%
• Metabolism: Partially hepatic to main metabolite, desethylchloroquine
• Excretion: Urine (≥50% as unchanged drug); acidification of urine increases elimination

Chloroquine has a very high volume of distribution, as it diffuses into the body's adipose tissue. Chloroquine and related quinines have been associated with cases of retinal toxicity, particularly when provided at higher doses for longer times. Accumulation of the drug may result in deposits that can lead to blurred vision and blindness. With long-term doses, routine visits to an ophthalmologist are recommended.

Chloroquine is also a lysosomotropic agent, meaning it accumulates preferentially in the lysosomes of cells in the body. The pK_a for the quinoline nitrogen of chloroquine is 8.5, meaning it is about 10% deprotonated at physiological pH as calculated by the Henderson-Hasselbalch equation. This decreases to about 0.2% at a lysosomal pH of 4.6. Because the deprotonated form is more membrane-permeable than the protonated form, a quantitative "trapping" of the compound in lysosomes results. (A quantitative treatment of this phenomenon involves the pK_as of all nitrogens in the molecule; this treatment, however, suffices to show the principle.)

The lysosomotropic character of chloroquine is believed to account for much of its antimalarial activity; the drug concentrates in the acidic food vacuole of the parasite and interferes with essential processes. Its lysosomotropic properties further allow for its use for in vitro experiments pertaining to intracellular lipid related diseases,^[25][26] autophagy, and apoptosis.^[27]

Mechanism of action

Antimalarial

Hemozoin formation in P. falciparum: many antimalarials are strong inhibitors of hemozoin crystal growth.

Inside red blood cells, the malarial parasite, which is then in its asexual lifecycle stage, must degrade hemoglobin to acquire essential amino acids, which the parasite requires to construct its own protein and for energy metabolism. Digestion is carried out in a vacuole of the parasitic cell.

Hemoglobin is composed of a protein unit (digested by the parasite) and a heme unit (not used by the parasite). During this process, the parasite releases the toxic and soluble molecule heme. The heme moiety consists of a porphyrin ring called Fe(II)-protoporphyrin IX (FP). To avoid destruction by this molecule, the parasite biocrystallizes heme to form hemozoin, a nontoxic molecule. Hemozoin collects in the digestive vacuole as insoluble crystals.

Chloroquine enters the red blood cell, inhibiting the parasite cell and digestive vacuole by simple diffusion. Chloroquine then becomes protonated (to CQ2+), as the digestive vacuole is known to be acidic (pH 4.7); chloroquine then cannot leave by diffusion. Chloroquine caps hemozoin molecules to prevent further biocrystallization of heme, thus leading to heme buildup. Chloroquine binds to heme (or FP) to form the FP-chloroquine complex; this complex is highly toxic to the cell and disrupts membrane function. Action of the toxic FP-chloroquine and FP results in cell lysis and ultimately parasite cell autodigestion. In essence, the parasite cell drowns in its own metabolic products.^[28] Parasites that do not form hemozoin are therefore resistant to chloroquine.^[29]

Other

Chloroquine inhibits thiamine uptake.^[30] It acts specifically on the transporter SLC19A3.

Against rheumatoid arthritis, it operates by inhibiting lymphocyte proliferation, phospholipase A2, antigen presentation in dendritic cells, release of enzymes from lysosomes, release of reactive oxygen species from macrophages, and production of IL-1.

Names

Brand names include Chloroquine FNA, Resochin, and Dawaquin.

History

Chloroquine was discovered in 1934 by Hans Andersag and coworkers at the Bayer laboratories, who named it "Resochin".^[31] It was ignored for a decade because it was considered too toxic for human use. During World War II, United States government-sponsored clinical trials for antimalarial drug development showed unequivocally that chloroquine has a significant therapeutic value as an antimalarial drug. It was introduced into clinical practice in 1947 for the prophylactic treatment of malaria.^[32]

Research

• Chloroquine is in clinical trials as an investigational antiretroviral in humans with HIV-1/AIDS and as a potential antiviral agent against chikungunya fever.^[33]
• The radiosensitizing and chemosensitizing properties of chloroquine are beginning to be exploited in anticancer strategies in humans.^[34][35]
• In biomedicinal science, chloroquine is used for in vitro experiments to inhibit lysosomal degradation of protein products.

See also

• History of malaria
• Quinine
• Artemether

References

External links

• Chloroquine Anti-HIV action
• Chloroquine and imatinib